![]() ![]() Images can also include draft genome assembly information to show read coverage, assembly breakpoints and collapsed repeats. The output image shows similarity between a central reference sequence and other sequences as a set of concentric rings, where BLAST matches are coloured on a sliding scale indicating a defined percentage identity. ResultsīLAST Ring Image Generator (BRIG) can generate images that show multiple prokaryote genome comparisons, without an arbitrary limit on the number of genomes compared. Currently, there is no freely available desktop application that enables users to rapidly visualise comparisons between hundreds of draft or complete genomes in a single image. Several programs, imaging libraries and internet resources already exist for this purpose, however, most are either limited in the number of comparisons they can show, are unable to adequately utilise draft genome sequence data, or require a knowledge of command-line scripting for implementation. Visualising a prokaryote genome as a circular image has become a powerful means of displaying informative comparisons of one genome to a number of others. New visualisation and abstraction methods are required in order to improve the validation, interpretation and communication of genome sequence information especially with the increasing amount of data arising from next-generation sequencing projects. ![]() Visualisation of genome comparisons is invaluable for helping to determine genotypic differences between closely related prokaryotes.
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